Overview
A prospective cohort study undergoing ICSI at a private fertility center in Egypt
Description
Purpose
To evaluate PGTai results for embryos cultured in sequential versus single step media.
MethodInjected oocytes will divided between Continuous Single Culture-NX media (CSC- NX) with 10% SSS the first group (Irvine, USA) and the other group will be cultured in sequential cleavage and blastocyst media with 10% SPS (SAGE, Denmark).
All blastocytes with 4BB quality and better according to Gardner's criteria 1999 will go through PGTA procedures with PGTai confirmation.
Eligibility
Inclusion Criteria:
- Female age 37 years with 8 mature oocytes
- Male partner had normal semen parameters according to WHO 2010
- PGT-A was done at the blastocyst stage
Exclusion Criteria:
- sperm or oocyte donor
- severe male factors according to WHO 2010